Gibberella zeae Ascospore Production and Collection for Microarray Experiments.

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Gibberella zeae ascospore production and collection for microarray experiments.

Fusarium graminearum Schwabe (teleomorph Gibberella zeae) is a plant pathogen causing scab disease on wheat and barley that reduces crop yield and grain quality. F. graminearum also causes stalk and ear rots of maize and is a producer of mycotoxins such as the trichothecenes that contaminate grain and are harmful to humans and livestock (Goswami and Kistler, 2004). The fungus produces two types...

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Some Genetic Techniques for Gibberella zeae

Leslie, J. F. 1983. Some genetic techniques for Gibberella zeae. Phytopathology 73:1005-1008. Gibberella zeae, an important plant pathogen, is exploited commercially dependent, glutamate dehydrogenase-deficient mutant. Protoplasts were in the production of zearalenone, a fungal sex hormone. Genetically, G. liberated by hyphal digestion with commercially available f-glucuronidase zeae is relativ...

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Mid1, a mechanosensitive calcium ion channel, affects growth, development, and ascospore discharge in the filamentous fungus Gibberella zeae.

The role of Mid1, a stretch-activated ion channel capable of being permeated by calcium, in ascospore development and forcible discharge from asci was examined in the pathogenic fungus Gibberella zeae (anamorph Fusarium graminearum). The Δmid1 mutants exhibited a >12-fold reduction in ascospore discharge activity and produced predominately abnormal two-celled ascospores with constricted and fra...

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Alignment of genetic and physical maps of Gibberella zeae.

We previously published a genetic map of Gibberella zeae (Fusarium graminearum sensu lato) based on a cross between Kansas strain Z-3639 (lineage 7) and Japanese strain R-5470 (lineage 6). In this study, that genetic map was aligned with the third assembly of the genomic sequence of G. zeae strain PH-1 (lineage 7) using seven structural genes and 108 sequenced amplified fragment length polymorp...

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ژورنال

عنوان ژورنال: Journal of Visualized Experiments

سال: 2006

ISSN: 1940-087X

DOI: 10.3791/115